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The double Olympic 800 metres champion had moved the Swiss Federal Tribunal to set aside a 2019 Court of Arbitration ruling that female athletes with high natural testosterone levels must take medication to reduce it.
The 28-year-old South African declared 'Hell no!' when asked if she would take testosterone-lowering medication.
Semenya, the double Olympic 800 metres champion, never declined an invitation to run in the Rabat 800m, her legal team said, and was told only on Friday that she was being invited to race having had an initial invitation revoked.
American Coleman, silver medallist in 2017 and owner of the fastest time this season, put down his gold marker by breezing to a time of 9.98 seconds to set the pace in the opening round.
The governing body of world athletics has hailed the Swiss court decision that ruled Caster Semenya out of the World Championships.
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