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Solicitor General Tushar Mehta, representing the government, stressed that the matter involved faith and that the use of contaminated ghee in preparing the laddus would be unacceptable.
YSRCP chief YS Jagan Mohan Reddy accused him of being a 'habitual liar' and wrote to Prime Minister Narendra Modi urging his intervention.
According to the Mayo Clinic, it is a white-coloured substance "Made by removing, simmering and clarifying the fatty tissue surrounding the organs of ruminant animals."
N Chandrababu Naidu claimed that animal fat was used to make Tirupati laddus during the previous Jagan Mohan Reddy government.
Official confirmation of the lab report is yet to come from the Andhra Pradesh government and the Tirumala Tirupati Devasthanams (TTD).
TTD plans to implement Facial Recognition based counter service to identify the pilgrim uniquely and to extend the service to the same person.
The development will benefit pilgrims from Kerala.
Videos of Nayanthara walking on the premises of the main temple with her footwear on and posing for photographs drew flak from various quarters.
Tirumala Tirupati Devasthanam (TTD), which manages the affairs of the country's richest temple, has 5,387 kg of gold with the State Bank of India and 1,938 kg of gold with the Indian Overseas Bank under gold deposit schemes.
Police said they have registered a case but no arrest has been made so far.