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The 22-year-old man had recently returned from the UAE where also his samples had tested positive for the disease.
Those who have travelled to a country where monkeypox has been reported in the last 21 days would be included in the list of persons to be kept under surveillance.
The disease is spread when people come into contact with the urine of rats, squirrel or cattle directly or indirectly.
All those who have been diagnosed with the disease hail from the Thirunelli area.
Two men from Wayanad were affected with Kyasanur Forest Disease (KFD), commonly called monkey fever.
Wayanad District Medical Officer Dr R Renuka told Onmanorama that two cases have been confirmed from Athattukunnu colony near Aranappara and Thonikkadav colony near Bavali in the district.
Last time the virus wreaked havoc in Wayanad was four years ago in 2015 when 102 cases were reported and 11 had died.