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Collins explained that she had been dealing with some health challenges over the last few months, including a diagnosis of endometriosis, and intimated that her plans to start a family had not proved as straightforward as she had hoped.
It's the first time in 15 years that an American man has reached a major final and both Fritz and Tiafoe wanted to be the one to break the barren streak.
Playing in her first major semifinal, Pegula was made to look like a novice by Muchova in a disastrous opening set, but somehow turned the tables on the unseeded Czech.
The 44-year-old Bopanna had also lost in the men's doubles.
The second-seeded Bopanna-Ebden pair lost to the 16th seed Argentine combo of Maximo Gonzalez and Andres Molteni in 66 minutes.
Djokovic will end the year without a major for the first time since 2017.
The former champion was stunned by Dutchman Botic van de Zandschulp in straight sets.
Osaka lost to Karolina Muchova in the second round, unable to get to grips with the Czech's superb serve-and-volley tennis.
The Italian defeated American Alex Michelsen 6-4 6-0 6-2.
The green-and-white outfit drew inspiration from Japan's colourful, playful and self-expressive Harajuku style.