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Earlier in the day, Audi announced it would be entering the sport in 2026. Audi are widely expected to join forces with Sauber.
The news was entirely expected and effectively confirmed when Bottas, 32, announced on Monday his switch to the Alfa Romeo team.
Raikkonen's participation at next weekend's Italian Grand Prix at Monza, the fastest track on the calendar and a home race for Alfa, is also in doubt as the Finn cannot return to the paddock until he receives a negative test.
Raikkonen, the 2007 world champion who is now 41, holds the records for most races entered (327) and started (324) by any driver in the history of the sport and is the oldest on the starting grid.
A spokesman for the governing body FIA said the International Court of Appeal in Paris had found the Swiss-based team's protest was not admissible.