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The department said symptoms such as fever, headache, body pain, joint pain, and red eyes should be taken seriously. It urged the public to promptly inform healthcare workers if they notice these symptoms.
The incubation period of the virus is three to 14 days and severe disease and deaths are rare, said District Medical Officer
The cold, cloudy weather and drizzles in the state due to the Mandous cyclone have further complicated the situation as the virus spread faster under these circumstances.
The finding could lead to the development and application of vector control strategies such as sugar baits, aimed at reducing arbovirus transmission.
The typical symptoms include fever, body ache and conjunctivitis.
With this, the total number down with the virus has gone up to 61 across the State.
The five who tested positive were residents of Anayara, Pettah, Karamana, Poojappura and Killipalam, respectively, in Thiruvananthapuram.
All three are from the Thiruvananthapuram district. Everyone's health is satisfactory and there are no concerns, Health Minister Veena George said.
None of the infected patients are admitted in the hospital and they all are stable.
Efforts to eradicate mosquito breeding grounds in the area have also been intensified.