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The federal agency announced that it has confiscated properties worth Rs 89.19 crore, which were seized in September 2020.
The accused cheated businessmen by offering fake foreign currency.
Ambani's statement was recorded as part of a fresh case filed under various sections of the Foreign Exchange Management Act.
The ED has also called for documents and the recording of statements of some company executives under provisions of the Foreign Exchange Management Act.
Global risk aversion has led to rupee depreciation against the dollar.
Accordingly, the Reserve Bank of India data showed that India's forex reserves fell to $622.275 billion from $631.920 billion reported the previous week.
Thampi has been charged by the agency in the past in alleged illegal hawala dealings and land purchase case in the country in 2017.
Travel industry sources said that Thomas Cook sends a lot of high-spending tourists to India from countries such as UK, Germany and France.
The overall forex reserves declined to $400.10 billion from $401.29 billion reported for the week ended August 24.
The reserve saw a rise in all other components including gold reserves, special drawing rights and the RBI's position with the IMF.